کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2425068 | 1552972 | 2007 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Characterization of Ca2+-ATPase activity in gill microsomes of freshwater mussel, Lamellidens marginalis (Lamarck) and heavy metal modulations Characterization of Ca2+-ATPase activity in gill microsomes of freshwater mussel, Lamellidens marginalis (Lamarck) and heavy metal modulations](/preview/png/2425068.png)
Freshwater mussel Lamellidens marginalis showed a Ca2+-stimulated ATPase activity in the microsomal fraction of gill. Mg2+ was also found to be an activating cation for this ATPase enzyme. Ca2+ ATPase showed maximal activity at 40 °C and between pH 7.5–8.0. Substrate specificity of Ca2+ ATPase was highest with ATP, followed by GTP and other trinucleotides such as UTP, CTP and ADP also showed some amount of hydrolysis. Ca2+ ATPase showed slight inhibition with ruthenium red and sodium vanadate and is insensitive to sodium azide, ouabain, oligomycin B and phenylalanine. Heavy metals like Hg2+, Cu2+ and Zn2+ showed 50% inhibition of Ca2+ ATPase activity at concentrations of 0.25 mM, 0.5 mM and 1 mM, respectively whereas Cd2+ and Ni2+ showed 39% and 28% inhibition of enzyme activity at 1 mM concentrations.
Journal: Aquaculture - Volume 270, Issues 1–4, 28 September 2007, Pages 443–450