کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2426227 1552988 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A new fibroblast like cell line from the fry of golden mahseer Tor putitora (Ham)
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
A new fibroblast like cell line from the fry of golden mahseer Tor putitora (Ham)
چکیده انگلیسی

We report the development of a diploid cell line (TP-1) for the first time from golden mahseer Tor putitora, a world famous food and game fish. Thirty-day-old fry were used to prepare a primary cell culture employing trypsin digestion. The optimal conditions of nutrition and incubation for growth of the dissociated cells were determined. The [3H] thymidine uptake assay was employed to investigate the effect of different concentrations of fetal calf serum (FCS) and fish muscle extract (FME) on the growth of TP-1 cells and to study the cell proliferation rate at different time intervals. It was found that L-15 supplemented with 20% FCS and 10% FME at an incubation temperature of 28 °C resulted in optimal growth. The concentration of fetal calf serum was reduced to 10% after 10 subcultures. After confluency, the cells were subcultured with a split ratio of 1:2. The cells showed a fibroblast-like morphology and reached confluency on the fourth day after subculture. The cells were successfully cryopreserved and revived at passage numbers 5, 8, 15 and 18. The cells were characterized for chromosome number (2n = 100) at 10 and 20 passages. The cell cycle analysis by FACS (Fluorescence Activated Cell Sorter) revealed that most of the cells on the first and fourth day of culture were in S-phase, indicating a high growth rate.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 253, Issues 1–4, 31 March 2006, Pages 238–243
نویسندگان
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