کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2430364 | 1106562 | 2007 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Breed effect on early cytokine mRNA expression in spleen and cecum of chickens with and without Salmonella enteritidis infection Breed effect on early cytokine mRNA expression in spleen and cecum of chickens with and without Salmonella enteritidis infection](/preview/png/2430364.png)
We examined mRNA expression of 11 genes: BAK, Bcl-x, Interferon [IFN]-γ, Interleukin [IL]-1β, IL-6, IL-10, IL-12α, IL-12β, IL-18, CXCLi2 [IL-8/CAF], and a MIP family chemokine, CCLi2, in the spleen and cecum of day-old chicks after oral inoculation with Salmonella enteritidis (SE) or medium. Three distinct chicken breeds (broiler, Fayoumi, and Leghorn) were evaluated for mRNA expression levels at 2 and 18 h post-inoculation using quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). SE exposure significantly increased splenic IL-18 and IFN-γ expression. Breed effect was significant (P<0.05)(P<0.05) for CXCLi2, IL-10, IL-12α, and CCLi2 mRNA expression in the spleen, and for IL-12α, IL-12β, IL-18, and CCLi2 mRNA expression in the cecum. Generally, mRNA expression levels were higher in the spleen, and lower in the cecum, of Leghorns versus broilers. These results support a role for breed genetics influencing cytokine mRNA expression in young chickens and may potentially explain some generalized immune response differences between breeds.
Journal: Developmental & Comparative Immunology - Volume 31, Issue 1, 2007, Pages 52–60