کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2430396 | 1106564 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
TNF induces the growth of thymocytes in rainbow trout
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شناسی تکاملی
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چکیده انگلیسی
In order to investigate the effects of TNFα upon the growth of fish thymocytes, rainbow trout thymocytes were cultured in the conditioned medium (CM): the supernatants of the macrophage cultures stimulated with chitin derivative and LPS. Synthesis of TNFα by macrophages and subsequent secretion into CM were ascertained by RT-PCR and western blotting. While most of the thymocytes cultured in normal medium died within 7 days, the thymocytes cultured in CM exhibited markedly better growth as monitored by alamarBlue⢠assay and BrdU assay. The proliferating cells appeared to be small lymphocytes. Since such activity in CM was significantly inhibited by an anti-trout TNF antibody, it was clearly evident that TNFα in the CM induced the proliferation of the thymocytes. Production of TNFα in the thymus of healthy fish was also demonstrated by RT-PCR. Collectively, this data suggest that TNFα is involved in T cell development in the trout thymus.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental & Comparative Immunology - Volume 30, Issue 7, 2006, Pages 639-647
Journal: Developmental & Comparative Immunology - Volume 30, Issue 7, 2006, Pages 639-647
نویسندگان
Kazuyoshi Hino, Osamu Nakamura, Yasutoshi Yoshiura, Hiroaki Suetake, Yuzuru Suzuki, Tasuku Watanabe,