کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2431269 1106750 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Systemic and mucosal immune response of rainbow trout to immunization with an attenuated Flavobacterium psychrophilum vaccine strain by different routes
ترجمه فارسی عنوان
پاسخ ایمنی سیستمیک و مخاطی ماهی قزل آلا رنگین کمان به ایمن سازی با سویه واکسن فلاواباکتریوم روانگردان با استفاده از روش های مختلف
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
چکیده انگلیسی


• Intraperitoneal immunization results in significant increase in IgM secretion in serum, gill mucus and skin mucus of rainbow trout.
• Tissues that were directly exposed to the bacterin, depending upon the immunization route, expressed the highest amount of IgM transcripts.
• Upregulation of IgT and IgD genes in mucosal tissues suggest that these two antibody isotypes are specialized mucosal antibodies.
• IgT and IgD offer early protection to the fish at mucosal surfaces against invading pathogens.

Teleosts possess three immunoglobulin (Ig) heavy chain isotypes viz., IgM, IgT and IgD and all three isotypes are reported in rainbow trout. The expression of these Ig isotypes in response to different immunization routes was investigated and results provide a better understanding of the role these Igs in different tissues. Rainbow trout (Oncorhynchus mykiss) were immunized with an attenuated Flavobacterium psychrophilum strain, 259-93-B.17 grown under iron limiting conditions, by intraperitoneal, anal intubation and immersion routes. Serum, gill mucus, skin mucus and intestinal mucus samples were collected at 0, 3, 7, 14, 28, 42 and 56 days post immunization by sacrificing four fish from each treatment group and the unimmunized control group, and the IgM levels were estimated by an enzyme linked immunosorbent assay (ELISA). In addition, blood, gill, skin and intestinal tissue samples were collected for Ig gene expression studies. The secretory IgM, IgD and IgT gene expression levels in these tissues were estimated by reverse transcription quantitative real time PCR (RT-qPCR). Levels of IgM in serum, gill and skin mucus increased significantly by 28 days after immunization in the intraperitoneally immunized group, while no significant increase in IgM level was observed in fish groups immunized by other routes. Secretory IgD and IgT expression levels were significantly upregulated in gills of fish immunized by the immersion route. Similarly, secretory IgT and IgD were upregulated in intestines of fish immunized by anal intubation route. The results confirm mucosal association of IgT and suggest that IgD may also be specialized in mucosal immunity and contribute to immediate protection to the fish at mucosal surfaces.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 44, Issue 1, May 2015, Pages 156–163
نویسندگان
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