کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2432057 | 1106778 | 2012 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Individual variability of mytimycin gene expression in mussel Individual variability of mytimycin gene expression in mussel](/preview/png/2432057.png)
The antifungal peptide mytimycin (MytM) is synthesized by hemocytes of the Mediterranean mussel, Mytilus galloprovincialis. In addition to sequence and gene structure diversities previously reported from pooled hemocytes, the present report focused on the expression of mytm gene in individual M. galloprovincialis, before and after challenge. Within untreated mussel, MytM mRNA was observed by ISH in about 42% of circulating hemocytes, characterized by large, diffuse nucleus. Injection with Fusarium oxysporum increased such percentage, but in only some of the mussels. Similarly, MytM gene expression increased after injection in only some of the mussels, as measured by qPCR. Responders and not responders are common evidence in any given population of organisms. Nevertheless, even if the use of proper pool size selection has been practised to find out and evaluate the most common response trends, individual analyses must be regarded as optimal.
► Antifungal mytimycin gene is expressed in 42% of circulating hemocytes in non-treated mussels.
► Injection with Fusarium oxysporum increased such percentage, as observed by ISH or measured in q-PCR.
► Only some mussels reacted to the injection.
Journal: Fish & Shellfish Immunology - Volume 33, Issue 3, September 2012, Pages 641–644