کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2432232 | 1106787 | 2011 | 8 صفحه PDF | دانلود رایگان |

The skin mucosal proteome of Atlantic cod (Gadus morhua) was mapped using a 2D PAGE, LC–MS/MS coupled approach. Mucosal proteins from naive fish were identified primarily by similarity searches across various cod EST databases. The identified proteins were clustered into 8 groups based on gene ontology classification for biological process. Most of the proteins identified from the gel are hitherto unreported for cod. Galectin-1, mannan binding lectin (MBL), serpins, cystatin B, cyclophilin A, FK-506 binding protein, proteasome subunits (alpha-3 and -7), ubiquitin, and g-type lysozyme are considered immune competent molecules. Five of the aforementioned proteins were cloned and their tissue distribution was analysed by RT-PCR.
► The skin mucosal proteome of naïve Atlantic cod (Gadus morhua) was characterised.
► Proteomics and bioinformatics identified proteins hitherto unreported for cod.
► Immune competent molecules were found in mucus.
► Immune competent molecules’ tissue distributions were analysed by RT-PCR.
Journal: Fish & Shellfish Immunology - Volume 31, Issue 2, August 2011, Pages 224–231