کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2432232 1106787 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Proteome reference map of the skin mucus of Atlantic cod (Gadus morhua) revealing immune competent molecules
چکیده انگلیسی

The skin mucosal proteome of Atlantic cod (Gadus morhua) was mapped using a 2D PAGE, LC–MS/MS coupled approach. Mucosal proteins from naive fish were identified primarily by similarity searches across various cod EST databases. The identified proteins were clustered into 8 groups based on gene ontology classification for biological process. Most of the proteins identified from the gel are hitherto unreported for cod. Galectin-1, mannan binding lectin (MBL), serpins, cystatin B, cyclophilin A, FK-506 binding protein, proteasome subunits (alpha-3 and -7), ubiquitin, and g-type lysozyme are considered immune competent molecules. Five of the aforementioned proteins were cloned and their tissue distribution was analysed by RT-PCR.


► The skin mucosal proteome of naïve Atlantic cod (Gadus morhua) was characterised.
► Proteomics and bioinformatics identified proteins hitherto unreported for cod.
► Immune competent molecules were found in mucus.
► Immune competent molecules’ tissue distributions were analysed by RT-PCR.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 31, Issue 2, August 2011, Pages 224–231
نویسندگان
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