کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2432370 1106792 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The first chordate big defensin: Identification, expression and bioactivity
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
The first chordate big defensin: Identification, expression and bioactivity
چکیده انگلیسی

Defensins are broadly present in plants, invertebrates and vertebrates, but little information is available about it in amphioxus, a protochordate holding a key phylogenetic position. In this study, a big defensin cDNA was identified from the amphioxus Branchiostoma japonicum (termed Bjbd). The cDNA contained an open reading frame (ORF) of 354 bp encoding a 117 amino acid protein, which had an N-terminal signal sequence followed by a propeptide and the mature big defensin. The mature peptide had the hydrophobic region GAAAVT(A)AA at N-terminus and the consensus pattern C-X6-C-X3-C-X13(14)-C-X4-C-C at C-terminus as well as four α-helices, four β-sheets, and three disulfide bridges (C1–C5, C2–C4 and C3–C6) in the predicted tertiary structure. This is the first big defensin gene ever identified in the phylum Chordata. Quantitative real-time PCR analysis revealed that Bjbd was constitutively expressed in most of the tissues examined, and its expression was remarkably up-regulated following the challenge with LPS, LTA, Aeromonas hydrophila and Staphylococcus aureus. Moreover, the recombinant BjBD was shown to be able to inhibit the growth of S. aureus, Escherichia coli and A. hydrophila. Taken together, these suggest that BjBD is a molecule involved in the removal of invading pathogens.


► The first big defensin gene Bjbd is identified in the phylum Chordata.
► Bjbd expression is markedly elevated after challenge with LPS and LTA and bacteria.
► Recombinant BjBD inhibits the growth of S. aureus, E. coli and A. hydrophila.
► BjBD is an anti-infection agent involved in the removal of invading pathogens.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 32, Issue 4, April 2012, Pages 572–577
نویسندگان
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