کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2436285 1107297 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gene deletion from Plasmodium falciparum using FLP and Cre recombinases: Implications for applied site-specific recombination
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی انگل شناسی
پیش نمایش صفحه اول مقاله
Gene deletion from Plasmodium falciparum using FLP and Cre recombinases: Implications for applied site-specific recombination
چکیده انگلیسی

The ability to manipulate the genome and induce site-specific recombination using either Flippase (FLP) or Cre recombinase has been useful in many systems including Plasmodium berghei for specific deletion events or to obtain conditional gene expression. To test whether these recombinases are active in Plasmodium falciparum we constructed gene knockouts that contain sequences recognised as templates for site-specific recombination. We tested the ability of FLP and Cre recombinases, expressed conditionally in P. falciparum, to mediate deletion of the human dihydrofolate reductase (hdhfr) drug resistance gene. We show that Cre recombinase is capable of efficient removal of hdhfr by site-specific recombination. In contrast, FLP recombinase is very inefficient, even at the optimum temperature of 30 °C for this enzyme. These results demonstrate that Cre recombinase can be utilised in P. falciparum for deletion of specific sequences such as drug resistance genes. This can be exploited for recycling of drug resistance cassettes and for the design of specific recombination events in P. falciparum.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal for Parasitology - Volume 41, Issue 1, January 2011, Pages 117–123
نویسندگان
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