کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2436824 | 1107359 | 2006 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reliable transfection of Plasmodium falciparum using non-commercial plasmid mini preparations
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موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
انگل شناسی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Transfection of Plasmodium falciparum is instrumental in the research on this parasite. However, the actual transfection protocol has not changed significantly since the first description and it is generally believed that large amounts of highly pure plasmid DNA are needed for successful transfection. Here, we report the transfection of P. falciparum using a protocol based on non-commercial mini-preparations of plasmid DNA. This method permits the reliable transfection of P. falciparum using less resources and lower costs, with a success rate comparable with currently used methods. A moderate throughput may be achieved using this method, providing a first step towards systematic transfection approaches in this parasite.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal for Parasitology - Volume 36, Issue 12, October 2006, Pages 1245–1248
Journal: International Journal for Parasitology - Volume 36, Issue 12, October 2006, Pages 1245–1248
نویسندگان
Tobias Spielmann, Matthew W.A. Dixon, Maria Hernandez-Valladares, Mandy Hannemann, Katharine R. Trenholme, Donald L. Gardiner,