کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2466412 1555333 2016 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Detection of bovine papillomavirus type 14 DNA sequences in urinary bladder tumors in cattle
ترجمه فارسی عنوان
تشخیص توالی DNA ویروس پاپیلومای نوع 14 گاو در تومورهای مثانه در گاو
کلمات کلیدی
Deltapapillomavirus گاو ؛ ویروس پاپیلومای نوع 14 گاو (BPV-14)؛ تومورهای مثانه؛ عفونت مشترک ویروس پاپیلومای
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
چکیده انگلیسی


• BPV-14 is associated with both urothelial and mesenchymal bladder tumors of cattle.
• This is the first study showing the presence of BPV-14 DNA in bladder tumors of cattle.
• This is the first report about the presence of BPV-14 in the European continent.
• BPV-14 is a novel bovine Deltapapillomavirus which has a worldwide distribution.

Bovine papillomavirus type 14 (BPV-14) is a novel Deltapapillomavirus (δPV) which is most closely related to BPV-1, -2, and -13, well-known members of the δPV genus. So far BPV-14 has been detected in cutaneous neoplastic lesions in cattle and in feline sarcoids. As BPV-14 may share biological and pathological properties with BPV-1, -2 and -13, it has been hypothesized that, like other δPVs, BPV-14 could be associated with bovine bladder neoplasia.In this study, 50 tumors of the urinary bladder of cattle were diagnosed. DNA was extracted from all tumor samples as well as from 25 normal bladder samples and submitted to BPV-14 L1 PCR and subsequent amplicon sequencing analysis. BPV-14 L1 DNA sequences of specific 195 bp amplicons were obtained from 17 of 50 (34%) tumor DNA isolates; no BPV-14 DNA was detected from 25 normal samples. Amplicons revealed a 99% homology with the corresponding BPV-14 L1 DNA region (GenBank accession number KP276343.1). Co-infections by two or three δPV types were also seen. This study reveals the presence of BPV-14 DNA alone or in combination with other δPV DNA in bovine bladder tumors alone and suggests that BPV-14 could also be involved in bladder neoplasia as its E5 oncoprotein has the potential to induce cell proliferation. Furthermore, this is the first study to show the presence of BPV-14 in Europe, suggesting that BPV-14, like other δPVs, has a worldwide distribution.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Microbiology - Volume 190, 15 July 2016, Pages 1–4
نویسندگان
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