کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2470523 1555733 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dicrocoelium dendriticum in cattle from Cypress Hills, Canada: Humoral response and preliminary evaluation of an ELISA
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Dicrocoelium dendriticum in cattle from Cypress Hills, Canada: Humoral response and preliminary evaluation of an ELISA
چکیده انگلیسی

Apparent changes in the distribution of Dicrocoelium dendriticum in western Canada and the absence of other liver flukes in the area prompted an examination of the humoral responses in naturally infected cattle sharing pasture with heavily infected wild cervids. Western blots using sera from the infected cattle indicated that the cattle had antibodies to various fluke antigens, however the pattern of response varied among isotypes. Isotype analysis indicated that Dicrocoelium-infected cattle produced IgG1, and IgM antibodies, but not IgG2 antibodies. Subsequent comparison of the efficiency of coprological and serological approaches for diagnosis showed that while 43.5% of a separate group of 26 yearlings had eggs in their faeces, the immunoblots indicated that all had detectable antibodies. A group of cattle necropsied for confirmation of fluke status was used to evaluate humoral immune responses and to provide baseline information in the preliminary development of an indirect ELISA for detection of anti-fluke antibodies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 174, Issues 1–2, 24 November 2010, Pages 162–165
نویسندگان
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