کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2471413 1555761 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
First molecular characterization of Babesia vogeli in two naturally infected dogs of Buenos Aires, Argentina
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
First molecular characterization of Babesia vogeli in two naturally infected dogs of Buenos Aires, Argentina
چکیده انگلیسی

Large piroplasms (>2.5 μm) were detected by direct microscopical investigation in 34 out of 16,767 (0.20%) canine blood smears in the Southern region of Greater Buenos Aires. Genomic DNA was extracted from two parasitemic dogs and the hypervariable 18S RNA gene region of the pathogen was specifically amplified, sequenced, and aligned with corresponding gene sequences available in the GenBank. Phylogenetic trees were constructed and compared. 18S RNA gene sequences reliably segregated in three clearly distinguishable clades representing Babesia canis, Babesia vogeli and Babesia rossi isolates, respectively. The 18S RNA gene sequences of both Babesia isolates from Argentina affiliated to the B. vogeli branch. This finding represents the first molecular evidence of the existence of B. vogeli in Argentina.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 157, Issues 3–4, 7 November 2008, Pages 294–298
نویسندگان
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