کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2471587 | 1555763 | 2008 | 4 صفحه PDF | دانلود رایگان |
This study was conducted to evaluate techniques for detection of small trematode eggs in faeces of dogs, cats and pigs. Faecal samples from dogs (n = 80), cats (n = 35) and pigs (n = 114) were examined by Kato-Katz technique (KK), formalin–ether sedimentation technique (FE) and a method of combining: filtration, sedimentation and centrifugation, developed at DBL – Centre for Health Research and Development (former Danish Bilharziasis Laboratory) (DBL). Necropsy was performed on 38 dogs, 25 cats and 16 pigs and was considered as a gold standard method for evaluation of the techniques. The results showed 100% specificity for the three techniques. Lower sensitivity was seen for the KK-technique in dog samples in comparison to that for DBL- and FE-technique. The sensitivity of the three techniques was similar in cats and pigs. Based on these findings and practical issues, DBL-technique was chosen as most suitable because the eggs were easily detected and quantified. No toxic chemicals or special equipment were required in comparison with FE-technique that needs ether solution and thus fume cupboards which are often unavailable in local veterinary centres in Vietnam and other developing countries.
Journal: Veterinary Parasitology - Volume 156, Issues 3–4, 1 October 2008, Pages 346–349