کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2471759 1555771 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular characterization of Babesia canis canis isolates from naturally infected dogs in Poland
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
Molecular characterization of Babesia canis canis isolates from naturally infected dogs in Poland
چکیده انگلیسی

Babesia canis has generally been considered the only large Babesia to infect dogs. In this study, we used PCR to detect and characterize B. canis canis isolated from naturally infected dogs in Poland by amplifying and sequencing a portion of the 18S ribosomal RNA (rRNA) gene. Venous blood samples were collected from 76 Babesia-symptomatic dogs. A 559-bp fragment of the B. canis canis 18S rRNA gene was amplified by PCR. The PCR products were then digested with HincII restriction enzyme, and isolates were classified according to whether they were cut (group A) or not (group B) by this endonuclease. Sequencing of the PCR products from the isolates led to the identification of seven sequence variants (four in group A, and three in group B). Sequences were compared with GenBank sequences, and alignments showed that all B. canis canis isolates from Europe may be classified into groups A or B as defined in our study.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Veterinary Parasitology - Volume 152, Issues 3–4, 15 April 2008, Pages 235–241
نویسندگان
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