کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2472610 | 1555795 | 2006 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Prevalence and genotyping of Cryptosporidium in three cattle husbandry systems in Zambia Prevalence and genotyping of Cryptosporidium in three cattle husbandry systems in Zambia](/preview/png/2472610.png)
To estimate the prevalence of Cryptosporidium spp. in three different husbandry systems in Zambia, faecal samples were collected from calves up to the age of 3 months. Faecal consistency was scored for correlation with infection. Additionally, 45 positive samples were selected for genotyping by amplification of the 70-kDa heat shock protein (HSP-70) and the 18S rRNA gene. A total of 37 dairy, 25 beef and 92 traditional husbandry farms were visited: 250 samples were collected on dairy farms, 238 on beef farms and 256 on traditional husbandry farms. All samples were analysed using a commercial copro-antigen ELISA (Techlab®Cryptospridium test). The calf prevalence in dairy, beef and traditional husbandry systems was 42.8%, 8.0% and 6.3%, respectively. Furthermore, 75.7% of the dairy farms, 44.0% of the beef farms and 15.2% of the traditional husbandry farms had at least one positive calf at the time of visit. Subsequently, there was a significantly higher Cryptosporidium parvum prevalence on dairy farms compared to beef or traditional farms (χ2, P < 0.001). On dairy farms low faecal consistency was correlated with C. parvum infection (χ2, P < 0.05). Both C. parvum and C. bovis were identified, although in one beef calf C. suis was found.
Journal: Veterinary Parasitology - Volume 138, Issues 3–4, 15 June 2006, Pages 217–222