Preliminary investigations into surface molecularly imprinted nanoparticles for Helicobacter pylori eradication

This paper reports investigations into the preparation and characterization of surface molecularly imprinted nanoparticles (SMINs) designed to adhere to Helicobacter pylori (H. pylori). Imprinted nanoparticles were prepared by the inverse microemulsion polymerization method. A fraction of Lpp20, an outer membrane protein of H. pylori known as NQA, was chosen as template and modified with myristic acid to facilitate its localization on the surface of the nanoparticles. The interaction between these SMINs with the template NQA were evaluated using surface plasmon resonance (SPR), change in zeta potential and fluorescence polarization (FP). The results were highly consistent in demonstrating a preferential recognition of the template NQA for SMINs compared with the control nanoparticles. In vitro experiments also indicate that such SMINs are able to adhere to H. pylori and may be useful for H. pylori eradication.

Surface molecularly imprinted nanoparticles (SMINs) designed to bind to Helicobacter pylori (H. pylori) have been prepared and characterized. Surface plasmon resonance, fluorescence polarization and changes in zeta potential were used to evaluate recognition between template and SMINs. In vitro experiments indicate nanoparticles imprinted with a amphiphilic template preferentially recognize H. pylori. SMINs appear to be a novel drug delivery system for eradication of H. pylori.Figure optionsDownload as PowerPoint slide