کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2484303 | 1114305 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transport Mechanism of Nicotine in Primary Cultured Alveolar Epithelial Cells
ترجمه فارسی عنوان
مکانیسم حمل و نقل نیکوتین در سلول های اپیتلیال آلوئولار اولیه کشت شده
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کلمات کلیدی
جذب، حمل و نقل فعال، فرهنگ سلولی، حمل و نقل غشاء / حمل کننده، تحویل / جذب ریوی،
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
چکیده انگلیسی
Nicotine is absorbed from the lungs into the systemic circulation during cigarette smoking. However, there is little information concerning the transport mechanism of nicotine in alveolar epithelial cells. In this study, we characterized the uptake of nicotine in rat primary cultured type II (TII) and transdifferentiated type I-like (TIL) epithelial cells. In both TIL and TII cells, [3H]nicotine uptake was time and temperature-dependent, and showed saturation kinetics. [3H]Nicotine uptake in these cells was not affected by Na+, but was sensitive to extracellular and intracellular pH, suggesting the involvement of a nicotine/proton antiport system. The uptake of [3H]nicotine in these cells was potently inhibited by organic cations such as clonidine, diphenhydramine, and pyrilamine, but was not affected by substrates and/or inhibitors of known organic cation transporters such as carnitine, 1-methyl-4-phenylpyridinium, and tetraethylammonium. In addition, the uptake of [3H]nicotine in TIL cells was stimulated by preloading the cells with unlabeled nicotine, pyrilamine, and diphenhydramine, but not with tetraethylammonium. These results suggest that a novel proton-coupled antiporter is involved in the uptake of nicotine in alveolar epithelial cells and its absorption from the lungs into the systemic circulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 105, Issue 2, February 2016, Pages 982-988
Journal: Journal of Pharmaceutical Sciences - Volume 105, Issue 2, February 2016, Pages 982-988
نویسندگان
Mikihisa Takano, Machi Nagahiro, Ryoko Yumoto,