کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2485521 1114357 2008 26 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Measurement of Dissociation Constants (pKa Values) of Organic Compounds by Multiplexed Capillary Electrophoresis Using Aqueous and Cosolvent Buffers*
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی اکتشاف دارویی
پیش نمایش صفحه اول مقاله
Measurement of Dissociation Constants (pKa Values) of Organic Compounds by Multiplexed Capillary Electrophoresis Using Aqueous and Cosolvent Buffers*
چکیده انگلیسی
Evaluation of a multiplexed capillary electrophoresis (CE) method for pKa measurements of organic compounds, including low solubility compounds, is presented. The method is validated on a set of 105 diverse compounds, mostly drugs, and results are compared to literature values obtained from multiple references. Two versions of the instrument in two different labs were used to collect data over a period of 3 years and inter-laboratory and inter-instrument variations are discussed. Twenty-four point aqueous and mixed cosolvent buffer systems were employed to improve the accuracy of pKa measurements. It has been demonstrated that the method allows direct pKa measurements in aqueous buffers for many compounds of low solubility, often unattainable by other methods. The pKa measurements of compounds with extremely low solubility using multiplexed CE with methanol/water cosolvent buffers are presented.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 97, Issue 7, July 2008, Pages 2581-2606
نویسندگان
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