کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2487272 | 1114410 | 2008 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A Comparison of the Effect of Chitosan and Chitosan-Coated Vesicles on Monolayer Integrity and Permeability Across Caco-2 and 16HBE14o-Cells
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
اکتشاف دارویی
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چکیده انگلیسی
The effects of chitosan-coated vesicles on the paracellular permeability of Caco-2 and 16HBE14o-cell lines, and their toxicity towards the same cell lines, have been compared to equivalent concentration of chitosan in solution. Chitosan-coated phospholipid vesicles and the same concentration of chitosan in solution were found to reduce the transepithelial electrical resistance (TER) of monolayers of 16HBE14o- and Caco-2 cells to a comparable extent. Upon removal of the vesicle suspension and the chitosan solution, TER had completely recovered within 24Â h for Caco-2 cells and to about 50% of its original value for the 16HBE14o-cells. The extent of enhancement of transport across 16HBE14o-cell monolayers of hydrophilic markers of varying molecular weight was found to be comparable in the presence of chitosan-coated phospholipid vesicles and the same concentration of chitosan and was dependent upon the molecular weight of the hydrophilic marker. Chitosan (either bound to vesicles or free in solution) did not display a significant toxicity towards the Caco-2 cell line whereas chitosan-coated phospholipid vesicles were less toxic towards the 16HBE14o-cell line than the equivalent chitosan concentration. These data suggest that chitosan-coated vesicles are good candidates for the delivery of drugs and other biomolecules across epithelial barriers.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical Sciences - Volume 97, Issue 9, September 2008, Pages 3998-4010
Journal: Journal of Pharmaceutical Sciences - Volume 97, Issue 9, September 2008, Pages 3998-4010
نویسندگان
L. Kudsiova, M.J. Lawrence,