کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2493810 1115528 2012 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Deltamethrin, a type II pyrethroid insecticide, has neurotrophic effects on neurons with continuous activation of the Bdnf promoter
موضوعات مرتبط
علوم زیستی و بیوفناوری علم عصب شناسی علوم اعصاب رفتاری
پیش نمایش صفحه اول مقاله
Deltamethrin, a type II pyrethroid insecticide, has neurotrophic effects on neurons with continuous activation of the Bdnf promoter
چکیده انگلیسی

Pyrethroids, widely used insecticides with low acute toxicity in mammals, affect sodium channels in neurons. In a primary culture of rat cortical neurons, deltamethrin (DM), a type II pyrethroid, markedly enhanced the expression of brain-derived neurotrophic factor (BDNF) exon IV-IX (Bdnf eIV-IX) mRNA. In this study, we found that DM has a neurotrophic effect on cultured neurons and investigated the mechanisms responsible for it. One μM DM increased cell survival, neurite complexity and length. Neurite complexity and length were reduced not only by a blockade of cellular excitation with GABA or Ca2+ influx via L-type voltage-dependent calcium channels with nicardipine, but also by a blockade of TrkB, a specific receptor for BDNF, with TrkB/Fc. These data indicate DM has neurotrophic actions. DM-induced Bdnf eIV-IX mRNA expression through the calcineurin and ERK/MAPK pathways, the increase of which was reduced by GABAA receptor activation. Using a promoter assay, we found that Ca2+-responsive elements including a CRE are involved in the DM-induced activation of the Bdnf promoter IV (Bdnf-pIV). The intracellular concentration of Ca2+ and activation of Bdnf-pIV remained elevated for, at least, 1 and 24 h, respectively. Moreover, GABAA receptor activation or a blockade of Ca2+ influx even after starting the incubation with DM reduced the elevated activity of Bdnf-pIV. These data demonstrated that the prolonged activation of Bdnf-pIV occurred because of this continuous increase in the intracellular Ca2+ concentration. Thus, DM has neurotrophic effects on neurons, likely due to prolonged activation of Bdnf promoter in neurons.This article is part of a Special Issue entitled ‘Post-Traumatic Stress Disorder’.


► Deltamethrin (DM) had neurotrophic effects on the survival and structure of neurons.
► DM maintained an elevated intracellular concentration of Ca2+ in neurons.
► DM triggered the prolonged activation of BDNF promoter IV (Bdnf-pIV).
► Prolonged DM-induced Bdnf-pIV activation was blocked by GABA.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuropharmacology - Volume 62, Issue 2, February 2012, Pages 1091–1098
نویسندگان
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