کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2514156 | 1118452 | 2008 | 8 صفحه PDF | دانلود رایگان |
Zinc (Zn2+) and copper (Cu2+) are key signalling molecules in the immune system and regulate the activity of many ion channels. Both Zn2+ and Cu2+ potently inhibit rat P2X7 receptors via a binding site identified by mutagenesis. Here we show that extracellular Cu2+ also potently inhibits mouse P2X7 receptors. By contrast, the receptor expression system and agonist strongly influence the action of extracellular Zn2+ at mouse P2X7 receptors. Consistent with previous reports, Zn2+ inhibits recombinant rat P2X7 receptors. However, recombinant mouse P2X7 receptors are potentiated by Zn2+ when activated by ATP4− but inhibited when stimulated with the ATP analogue BzATP4−. Endogenous murine macrophage P2X7 receptors are not modulated by Zn2+ when stimulated by ATP4− however Zn2+ inhibits BzATP4− mediated responses. In summary, these findings provide a fundamental insight into the differential actions of Zn2+ and Cu2+ between different P2X7 receptor species.
Journal: Biochemical Pharmacology - Volume 76, Issue 12, 15 December 2008, Pages 1740–1747