کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2514328 1118462 2009 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Antihypertensive and vasorelaxant effects of tilianin isolated from Agastache mexicana are mediated by NO/cGMP pathway and potassium channel opening
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی داروشناسی
پیش نمایش صفحه اول مقاله
Antihypertensive and vasorelaxant effects of tilianin isolated from Agastache mexicana are mediated by NO/cGMP pathway and potassium channel opening
چکیده انگلیسی

Current investigation was undertaken to elucidate the mode of action of tilianin, isolated from Agastache mexicana, as a vasorelaxant agent on in vitro functional rat thoracic aorta test and to investigate the in vivo antihypertensive effect on spontaneously hypertensive rats (SHR). Tilianin (0.002–933 μM) induced significant relaxation in a concentration- and endothelium-dependent and -independent manners in aortic rings pre-contracted with noradrenaline (NA, 0.1 μM), and serotonin (5-HT, 100 μM). Effect was more significant (p < 0.05) in endothelium-intact (+E) aorta rings than when endothelium was removed (−E). Pre-treatment with N-nitro-l-arginine methyl ester (l-NAME; 10 μM) or 1-H-[1,2,4]-oxadiazolo-[4,3a]-quinoxalin-1-one (ODQ, 1 μM) produced a significant change of the relaxant response and activity was markedly inhibited, but not by indomethacin (10 μM) or atropine (1 μM). Furthermore, tilianin (130 μM) provoked a significant displacement to the left in the relaxation curve induced by sodium nitroprusside (SNP; 0.32 nM to 0.1 μM). Moreover, tilianin induced significant in vitro NO overproduction (1.49 ± 0.86 μM of nitrites/g of tissue) in rat aorta compared with vehicle (p < 0.05). In addition, pre-treatment with tetraethylammonium (TEA, 5 mM) and 2-aminopyridine (2-AP, 0.1 μM) shifted to the right the relaxant curve induced by tilianin (p < 0.05). Finally, a single oral administration of tilianin (50 mg/kg) exhibited a significant decrease in systolic and diastolic blood pressures (p < 0.05) in SHR model.Results indicate that tilianin mediates relaxation mainly by an endothelium-dependent manner, probably due to NO release, and also through an endothelium-independent pathway by opening K+ channels, both causing the antihypertensive effect.

Tilianin mediates relaxation by an endothelium-dependent manner, probably due to NO release, and also through an endothelium-independent pathway by opening K+ channels, both causing the antihypertensive effect.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 78, Issue 1, 1 July 2009, Pages 54–61
نویسندگان
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