کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2514724 1118480 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of zebrafish histamine H1, H2 and H3 receptors and effects of histaminergic ligands on behavior
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی داروشناسی
پیش نمایش صفحه اول مقاله
Identification of zebrafish histamine H1, H2 and H3 receptors and effects of histaminergic ligands on behavior
چکیده انگلیسی

Neuronal histamine regulates several functions in the vertebrate brain. The zebrafish brain contains a widespread histaminergic system and H3 receptor ligand binding has been reported. In this study we provide evidence for the existence of histamine H1, H2 and H3 receptor genes in zebrafish. Single copies of putative histamine H1, H2 and H3 receptors were identified and cloned from the zebrafish brain. Expression analysis suggested that they are expressed in the brain and a few other tissues. Widespread distribution of zebrafish H2 receptor binding sites was detected with [125I]iodoaminopotentidine in brain sections. Zebrafish larvae were exposed to 1, 10 or 100 μM of the H1 ligand pyrilamine, the H2 ligand cimetidine and the H3 ligands thioperamide and immepip for 5 days. Significant decreases in swimming distance were observed with the highest dose of all ligands, whereas cimetidine gave a significant decrease also with 1 and 10 μM doses. These results provide the first molecular biological evidence for the presence of histamine receptors in zebrafish. These histamine receptors resemble those of higher vertebrates and they provide a useful model for pharmacological and behavioral studies for characterizing the functions of histamine in more detail.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 73, Issue 8, 15 April 2007, Pages 1205–1214
نویسندگان
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