کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2514842 | 1118488 | 2007 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Involvement of p38MAPK on the antinociceptive action of myricitrin in mice Involvement of p38MAPK on the antinociceptive action of myricitrin in mice](/preview/png/2514842.png)
Previous studies from our group investigated the analgesic and anti-inflammatory properties of the flavonoid myricitrin. Here, we demonstrated the role of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and mitogen-activated protein kinases (MAPKs) on the antinociceptive action of myricitrin. The nociceptive response was evaluated by monitoring biting behaviour following intratecal (i.t.) administration of IL-1β and TNF-α in mice. Western blot analyses of total and phosphorylated MAPKs: p38MAPK, extracellular-signal regulated kinase (ERK1/2) and c-Jun amino-terminal kinases (JNK1/2) from the spinal cord of mice injected with cytokines were measured. Myricitrin (0.03–30 mg/kg) or vehicle (control) was administered 30 min beforehand by intraperitoneal (i.p.) injection. Myricitrin pre-treatment prevented cytokine-induced biting behaviour. The calculated ID50 of myricitrin were 6.8 (4.6–9.0) and 2.6 (0.3–4.9) mg/kg and maximal inhibition of 83 ± 9 and 100 ± 0% for IL-1β and TNF-α, respectively. Intrathecal injection of IL-1β and TNF-α significantly increased p38MAPK phosphorylation and this was inhibited by myricitrin treatment. Cytokines administration did not alter ERK1/2 and JNK1/2 phosphorylation. Myricitrin prevented cytokine-induced biting behaviour and inhibited p38MAPK phosphorylation in response to cytokines stimulation. Taken together, it suggests that the mechanism for antinociceptive action of myricitrin in response to cytokines may involve a blockage on p38MAPK pathway. This finding could explain, at least in part, the antinociceptive action of this flavonoid in process like neuropathic and inflammatory chronic pain.
Journal: Biochemical Pharmacology - Volume 74, Issue 6, 15 September 2007, Pages 924–931