کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2514952 | 1118494 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Conformational state of human cardiac 5-HT4(g) receptors influences the functional effects of polyclonal anti-5-HT4 receptor antibodies
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کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The functional effects of the anti-G21V antibody directed against the second extracellular loop of human heart 5-HT4 receptors can differ when the receptors are expressed in different cell lines. Here, we extend these studies to show variation in the responses of 5-HT4(g) receptors to the antibody within the same expression system. In a previous report no effect of the anti-G21V antibodies had been shown upon 5-HT4(g) receptors expressed in CHO cells. Here the same antibodies alone or when added before 5-HT had a functional “inverse-agonist like” effect upon 5-HT4(g) receptors expressed in a separate line of CHO cells. Although these CHO cells showed a lower efficacy of cAMP production evoked by 5-HT than the previous report they express a similar h5-HT4(g) receptor density. Inhibition of either phosphodiesterases or Gi proteins had no effect upon the action of the antibody. Conformational states of the 5-HT4 receptor and/or equilibrium between different states of receptors may then determine the functional effect of antibodies against this receptor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 73, Issue 7, 1 April 2007, Pages 964-971
Journal: Biochemical Pharmacology - Volume 73, Issue 7, 1 April 2007, Pages 964-971
نویسندگان
Emmanuella Di Scala, Stéphanie Rose, Olivier Hérault, Jorge Argibay, Pierre Cosnay, Véronique Bozon,