کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2516028 1118624 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Differentiation of PC12 cells induced by N8-acetylspermidine and by N8-acetylspermidine deacetylase inhibition
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی داروشناسی
پیش نمایش صفحه اول مقاله
Differentiation of PC12 cells induced by N8-acetylspermidine and by N8-acetylspermidine deacetylase inhibition
چکیده انگلیسی

Spermidine is one of the simple polyamines found in cells of virtually all living organisms. It undergoes a metabolic conversion to N8-acetylspermidine catalyzed by an enzyme in cell nuclei and is converted back to spermidine by a deacetylase in the cytoplasm. In this study, two different mechanisms were used to produce an elevation in the level of N8-acetylspermidine in PC12 cells: inhibition of N8-acetylspermidine deacetylase and direct addition of N8-acetylspermidine to the cell culture. The increasing intracellular concentration of N8-acetylspermidine was accompanied by signs of PC12 cell differentiation including increased content of dopamine and morphological changes (neurite outgrowths), suggesting a strong and perhaps causal relationship among these effects. This effect on differentiation appears to be specific for N8-acetylspermidine as the addition of other polyamines including spermidine and N1-acetylspermidine did not elicit these changes. Nerve growth factor (NGF) and dexamethasone, commonly used inducers of differentiation in PC12 cells, produced differentiation without measurable changes in N8-acetylspermidine levels, suggesting that different (or multiple) mechanisms may be involved in these differentiation processes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 63, Issue 11, 1 June 2002, Pages 2011–2018
نویسندگان
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