Association of seven thrombotic pathway gene CpG-SNPs with coronary heart disease

ObjectivesCoronary heart disease (CHD) has been considered a thromboembolic arterial diseases. The aim of this case–control study was to explore whether the CpG-SNPs of the thrombotic pathway genes contributed to the risk of CHD.Methods and materialsA total of 784 CHD patients and 738 healthy controls were recruited in the current association study, which evaluated 7 CpG-SNPs of the thrombotic pathway genes. The CpG-SNPs included THBS4 rs17878919, CYP2C19 rs12773342, P2RY12 rs1491974, ITGA2 rs26680, FGB rs2227389, F7 rs510317 and F5 rs2269648. SNP genotyping was performed with a Sequenom Mass Spectrometry Genetic Analyzer.ResultsOur results demonstrated that CYP2C19 rs12773342 polymorphism was significantly associated with CHD in the recessive model (χ2 = 5.41, df = 1, P = 0.020, OR = 1.455, 95% CI = 1.060–1.996). A breakdown analysis by age showed that the association of CYP2C19 rs12773342 with CHD was mainly found in individuals aged 55–65 (genotype: χ2 = 7.93, df = 2, P = 0.019; allele: χ2 = 4.45, df = 1, P = 0.035). In addition, we also observed a significant association between F7 rs510317 polymorphism and CHD in males (genotype: χ2 = 7.24, df = 2, P = 0.027). There was no significant association with CHD for the remaining CpG-SNPs.ConclusionOur results supported that the CYP2C19 rs12773342 and F7 rs510317 polymorphisms were associated with CHD in the Han Chinese population.