کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2524083 1557971 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Anti-fibrotic effect of trans-resveratrol on pancreatic stellate cells
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی تومور شناسی
پیش نمایش صفحه اول مقاله
Anti-fibrotic effect of trans-resveratrol on pancreatic stellate cells
چکیده انگلیسی


• The natural stilbenoid resveratrol possesses potent anti-fibrotic activities.
• Resveratrol suppressed expression level of alpha-smooth muscle actin.
• Resveratrol reduced expression levels of collagen I and fibronectin.

Trans-resveratrol, also known as 3,5,4′-trihydroxy-trans-stilbene, is a natural stilbenoid found at high concentration in skins of red grapes and berries. Over the recent years, it has been reported with a variety of beneficial effects such as antioxidant, anti-aging and anti-inflammatory bioactivities; thus often utilized as an active substance in human and veterinary therapeutics. In the current study, we aimed to delineate the mechanism of its anti-fibrotic action by means of various biochemical assays, such as immunofluorescent staining, real-time polymerase chain reaction and Western blotting analyses in a cellular model, the LTC-14 cells, which retain essential characteristics and morphological features of primary pancreatic stellate cells (PSCs). Our results demonstrated that the application of trans-resveratrol as low as 10 μM notably suppressed the mRNA and protein levels of different fibrotic mediators namely alpha-smooth muscle actin, type I collagen and fibronectin in the LTC-14 cells stimulated with transforming growth factor-beta, a well recognized pro-fibrotic inducer. Importantly, the mechanism of the anti-fibrotic action of trans-resveratrol was associated with a decrease in nuclear factor-kappaB activation and protein kinase B phosphorylation. In conclusion, our finding suggests that trans-resveratrol may serve as a therapeutic or an adjuvant agent in anti-fibrotic approaches and/or PSC-relating pathologies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 71, April 2015, Pages 91–97
نویسندگان
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