کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2524272 1119552 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Smoothened activates breast cancer stem-like cell and promotes tumorigenesis and metastasis of breast cancer
ترجمه فارسی عنوان
استحکام سلول های ساقه مانند سرطان پستان را فعال می کند و باعث تومور زایی و متاستاز سرطان پستان می شود
کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی تومور شناسی
چکیده انگلیسی

Smoothened (Smo) is a G protein-coupled receptor protein encoded by the Smo gene of the hedgehog signalling pathway, which is thought to play an important role in maintaining organ patterning, cell differentiation and self-renewal. The possible role of Smo in the process of tumorigenesis and metastasis of breast cancer still remains unclear. The present experiments were to investigate the effect of Smo on activating breast cancer stem-like CD44+CD24− cells and the tumorigenesis and metastasis of breast cancer. By injected CD44+CD24− cells (1 × 104) into the cleared fat pad of NOD/SCID mice, it was observed that CD44+CD24− cells possess higher tumor-initiating capacity and metastasis properties than equal numbers of non-CD44+CD24− cells. The mRNA and protein expressions of Smo in CD44+CD24− cells were higher than those in non-CD44+CD24− cells, indicating that Smo may play a role in maintaining breast cancer stem cell features. qRT–PCR results revealed that expressions of STAT3, Bcl-2 and cyclinD1 mRNA in MCF-7 cells were decreased after transfected by Smo siRNA. In addition, the expressions of MMP-2 and MMP-9 were downregulated in MCF-7 cells after Smo expression was inhibited. Smo inhibition may be a possible therapeutic target that potentially suppresses breast tumor formation and development.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 68, Issue 8, October 2014, Pages 1099–1104
نویسندگان
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