کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2524391 | 1119564 | 2012 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Trichostatin A reduced phospholipase C gamma-1 transcript and protein contents in MCF-7 breast cancer cells Trichostatin A reduced phospholipase C gamma-1 transcript and protein contents in MCF-7 breast cancer cells](/preview/png/2524391.png)
It has recently been demonstrated that phospholipase C gamma-1 (PLCγ1) activation may contribute to breast carcinoma cell motility and their metastasis. Employing MCF-7 breast cancer cells, we showed the effect of trichostatin A (TSA) on the cellular contents of the PLCγ1 molecule. Using reverse transcription, real-time quantitative PCR and western blot analysis, we demonstrated that TSA reduced the PLCγ1 transcript and protein levels in MCF-7 cells. We also found that TSA decreased the half-life of the PLCγ1 transcript from approximately 7 hours to 5 hours. Moreover, we observed that protein synthesis appears to be essential in the TSA reduction of PLCγ1 mRNA stability. Since PLCγ1 activation is considered a key factor in the initiation of events that increase malignant cell motility, our observations may support the validity of TSA in anticancer studies.
Journal: Biomedicine & Pharmacotherapy - Volume 66, Issue 1, February 2012, Pages 1–5