کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2525228 1119603 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Trichostatin A down-regulates CYP19 transcript and protein levels in MCF-7 breast cancer cells
کلمات کلیدی
موضوعات مرتبط
علوم پزشکی و سلامت پزشکی و دندانپزشکی تومور شناسی
پیش نمایش صفحه اول مقاله
Trichostatin A down-regulates CYP19 transcript and protein levels in MCF-7 breast cancer cells
چکیده انگلیسی

Epidemiological and experimental evidence implicates estrogens in the etiology and progression of breast cancer. The biosynthesis of estrogens from androgens is catalyzed by an enzymatic complex designated as aromatase (CYP19).Using quantitative real-time PCR and Western blot analysis, we demonstrated that trichostatin A (TSA) histone deacetylase inhibitor significantly reduced CYP19 transcript and protein contents in MCF-7 breast cancer cells. We also found that TSA lowered CYP19 transcript stability and significantly decreased the transcript's half-life from approximately 6 h to 3.5 h. Our results from experiments with a protein biosynthesis inhibitor suggest the involvement of an RNase and/or mRNA stabilization protein in CYP19 transcript stabilization.Since malignant tissue aromatase is a significant estrogen producer involved in breast tumor progression, our findings may have clinical implication.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 63, Issue 4, May 2009, Pages 262–266
نویسندگان
, ,