کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2540160 1559750 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Methionine enkephalin regulates microglia polarization and function
ترجمه فارسی عنوان
متیونین انکافالین، قطبی شدن و عملکرد میکروگلوی را تنظیم می کند
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
چکیده انگلیسی


• MENK therapy induced up-regulation of CD86, CD40, IL-12, and TNF-α, but had no effect on CD163, IL-10, and TGF-β.
• A high phagocytosis capacity and increased expression of ROS, nitrite and iNOS were observed in MENK-treated microglia.
• Supernatants from MENK-treated microglia induced cytotoxicity against glioblastoma cells.

Methionine enkephalin (MENK), an opioid peptide, is known to function as a regulator in the immune system. As microglia are considered the most important immune cells in the central nervous system (CNS), we aimed to assess the function of MENK on microglia polarization and tumoricidal responses. Initially, we chose the most optimal condition of 10− 12 M for 48 h; however, MENK had no function on the viability and apoptosis of microglia under this treatment. However, MENK treatment markedly increased levels of M1-associated genes, such as CD86, CD40, IL-12, and TNF-α, but had no effect on M2 markers, including CD163, IL-10, and TGF-β. Moreover, microglia in the MENK-treated group showed high phagocytosis capacity, which coincided with characteristics of M1 microglia. MENK stimulation also induced up-regulation of reactive oxygen species (ROS) expression, which contributed to maintaining homeostasis. We also detected NO production by measuring the end product nitrite, and found that MENK treatment increased expression of nitrite and inducible NO synthase (iNOS), but did not influence arginase-1 (Arg1) expression. Furthermore, treatment of microglia with MENK led to a significant increase in cytotoxicity against glioblastoma cells, indicating that MENK possessed anti-tumor ability. Overall, MENK treatment could induce microglia to an M1 phenotype, modulating Th1 responses in the immune system. Additionally, microglia treated with MENK had tumoricidal activity, which provides new insight into anti-tumor immunity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 40, November 2016, Pages 90–97
نویسندگان
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