کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2540359 1559760 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The anti-inflammatory effect of TR6 on LPS-induced mastitis in mice
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
The anti-inflammatory effect of TR6 on LPS-induced mastitis in mice
چکیده انگلیسی


• TR6 significantly inhibited MPO activity, and LPS-induced production of TNF-α, IL-1β and IL-6.
• TR6 significantly inhibited LPS-induced TNF-α and IL-6 production.
• TR6 significantly inhibited LPS-induced NF-κB and MAPKs activation.

[TRIAP]-derived decoy peptides have anti-inflammatory properties. In this study, we synthesized a TRIAP-derived decoy peptide (TR6) containing, the N-terminal portion of the third helical region of the [TIRAP] TIR domain (sequence “N”-RQIKIWFQNRRMKWK and -KPGFLRDPWCKYQML-“C”). We evaluated the effects of TR6 on lipopolysaccharide-induced mastitis in mice. In vivo, the mastitis model was induced by LPS administration for 24 h, and TR6 treatment was initiated 1 h before or after induction of LPS. In vitro, primary mouse mammary epithelial cells and neutrophils were used to investigate the effects of TR6 on LPS-induced inflammatory responses. The results showed that TR6 significantly inhibited mammary gland hisopathologic changes, MPO activity, and LPS-induced production of TNF-α, IL-1β and IL-6. In vitro, TR6 significantly inhibited LPS-induced TNF-α and IL-6 production and phosphorylation of NF-κB and MAPKs. In conclusion, this study demonstrated that the anti-inflammatory effect of TR6 against LPS-induced mastitis may be due to its ability to inhibit TLR4-mediated NF-κB and MAPK signaling pathways. TR6 may be a promising therapeutic reagent for mastitis treatment.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 30, January 2016, Pages 150–156
نویسندگان
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