کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2541052 | 1122638 | 2011 | 7 صفحه PDF | دانلود رایگان |

Recent studies have shown that indoleamine 2,3-dioxygenase (IDO) plays a pivotal role in the modulation of immune response against tumor and virus infection. Here we demonstrate the pro-apoptotic effect of l-kynurenine, a tryptophan catabolite of IDO, on human NK cell line, NK92 MI. Treatment with l-kynurenine dose-dependently induced growth inhibition and apoptosis in NK92 MI cells. Treatment with the antioxidant NAC completely protected cells from l-kynurenine-induced apoptosis. Moreover, we found that treatment with Z-VAD-fmk and ZB4 slightly inhibited l-kynurenine-induced apoptosis, suggesting that l-kynurenine-induced apoptosis in NK cells occurs primarily through an ROS mediated pathway. We observed that the presence of NAC blocks cytochrome c release and activation of caspase-3 during l-kynurenine-induced apoptosis. Overall, we conclude that l-kynurenine resulting from IDO can cause cell death via ROS pathway in NK cells. Our findings provide a new insight into the interaction between NK cells and IDO positive cancer cells in regulating immune responses.
Journal: International Immunopharmacology - Volume 11, Issue 8, August 2011, Pages 932–938