کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2541393 | 1559762 | 2010 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Anti-inflammatory effects of cordycepin via suppression of inflammatory mediators in BV2 microglial cells Anti-inflammatory effects of cordycepin via suppression of inflammatory mediators in BV2 microglial cells](/preview/png/2541393.png)
Cordyceps militaris, a traditional medicinal mushroom, produces the bioactive compound cordycepin (3'-deoxyadenosine). Although cordycepin has been shown to have pharmacological, immunological stimulating, anti-cancer, and anti-inflammatory activities, its activities and cellular mechanisms during microglial activation have yet to be elucidated. Thus, we evaluated the anti-inflammatory effect of cordycepin on the production of inflammatory mediators in lipopolysaccharide (LPS)-stimulated murine BV2 microglia. We also investigated the effects of cordycepin on LPS-induced nuclear factor-kappaB (NF-κB) activation and on phosphorylation of mitogen-activated protein kinases (MAPKs). After LPS stimulation, nitric oxide (NO), prostaglandin E2 (PGE2), and pro-inflammatory cytokine production was detected in BV2 microglia. However, we found that cordycepin significantly inhibited the excessive production of NO, PGE2, and pro-inflammatory cytokines in a concentration-dependent manner without causing cytotoxicity. In addition, cordycepin suppressed NF-κB translocation by blocking IkappaB-α (IκB-α) degradation and inhibited the phosphorylation of Akt, ERK-1/2, JNK, and p38 kinase. Our results indicate that the inhibitory effect of cordycepin on LPS-stimulated inflammatory mediator production in BV2 microglia is associated with the suppression of the NF-κB, Akt, and MAPK signaling pathways. Therefore, cordycepin may be useful in treating neurodegenerative diseases by inhibiting inflammatory mediator production in activated microglia.
Research Highlights
► Cordycepin exert an anti-inflammatory effect on the BV2 microglia cell line.
► Cordycepin significantly attenuated the release of inflammatory mediators.
► Cordycepin inhibit activation of NF-kB, Akt, and MAPKs.
Journal: International Immunopharmacology - Volume 10, Issue 12, December 2010, Pages 1580–1586