کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2541842 1122677 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nicotine induces cyclooxygenase-2 and prostaglandin E2 expression in human umbilical vein endothelial cells
موضوعات مرتبط
علوم زیستی و بیوفناوری ایمنی شناسی و میکروب شناسی ایمونولوژی
پیش نمایش صفحه اول مقاله
Nicotine induces cyclooxygenase-2 and prostaglandin E2 expression in human umbilical vein endothelial cells
چکیده انگلیسی

Nicotine is a major component of cigarette smoking which may be involved in the progress of atherogenesis. In order to explain the mechanism of nicotine-induced endothelium dysfunction, we investigated the effects of nicotine on cyclooxygenase-2 (COX-2) and intercellular adhesion molecule-1 (ICAM-1) expression in human umbilical vein endothelial cells (HUVECs). Nicotine treatment increased the expressions of COX-2 at mRNA and protein level in a dose-dependent manner, following prostaglandin E2 (PGE2) release enhancement. Pyrrolidine dithiocarbamate (PDTC, NF-κB inhibitor) and α-Bungarotoxin (α-Btx, nicotinic acetylcholine receptor antagonist) attenuated the nicotine-induced COX-2 expression and PGE2 production. Furthermore, nicotine-induced ICAM-1 expression was reduced by NS-398 (selective COX-2 inhibitor). Taken together, the present study demonstrated that nicotine-induced COX-2 expression through NF-κB activation which mediated by nicotinic acetylcholine receptor and the induction of COX-2 was related to ICAM-1 expression.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Immunopharmacology - Volume 10, Issue 4, April 2010, Pages 461–466
نویسندگان
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