کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2544844 1560377 2016 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
1H NMR and MS based metabolomics study of the therapeutic effect of Cortex Fraxini on hyperuricemic rats
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی داروشناسی
پیش نمایش صفحه اول مقاله
1H NMR and MS based metabolomics study of the therapeutic effect of Cortex Fraxini on hyperuricemic rats
چکیده انگلیسی

Ethnopharmacological relevanceCortex Fraxini (CF) is an important traditional Chinese herbal medicine used for the treatment of gout and hyperuricemia.Aim of the studyThe aim of this study was to evaluate the anti-hyperuricemic effect of CF on hyperuricemic rats and to investigate its mechanism of action.Materials and methodsMetabolomics based on NMR and MS was used to study the therapeutic effect of CF on hyperuricemic rats. Plasma determination of uric acid (UA) showed that CF treatment markedly improved the UA level. Subsequently, metabolomics analysis was conducted using samples of plasma, kidney and urine, and orthogonal partial least squares-discriminant analysis (OPLS-DA) combined with principal component analysis (PCA) were used to detect potential biomarkers.ResultsA total of 26 biomarkers were identified as being primarily involved in amino acid metabolism, lipid metabolism, purine metabolism, amino acid metabolism and carbohydrate metabolism, and hyperuricemia can disturb the balance of many of these metabolic pathways in vivo.ConclusionsThe variations in biomarkers revealed the therapeutic mechanism of CF, and a number of these biomarkers are not only significant for early diagnosis but also for predicting hyperuricemia.

Figure optionsDownload high-quality image (212 K)Download as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Ethnopharmacology - Volume 185, 5 June 2016, Pages 272–281
نویسندگان
, , , , , ,