کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2545283 | 1123945 | 2014 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Inhibition of 5-lipoxygenase as anti-inflammatory mode of action of Plectranthus zeylanicus Benth and chemical characterization of ingredients by a mass spectrometric approach Inhibition of 5-lipoxygenase as anti-inflammatory mode of action of Plectranthus zeylanicus Benth and chemical characterization of ingredients by a mass spectrometric approach](/preview/png/2545283.png)
Ethnopharmacological relevanceThe perennial herb Plectranthus zeylanicus Benth is extensively used in traditional medicine in Sri Lanka and South India for treating inflammatory conditions, but pharmacological features of Plectranthus zeylanicus are hardly explored in order to understand and rationalize its use in ethnomedicine. As 5-lipoxygenase (5-LO) is a key enzyme in inflammatory disorders such as asthma or atherosclerosis, we investigated 5-LO inhibition by Plectranthus zeylanicus extracts and analyzed relevant constituents.Materials and methodsWe applied cell-free and cell-based assays to investigate suppression of 5-LO activity. Cell viability, radical scavenger activities, and inhibition of reactive oxygen species formation (ROS) in neutrophils were analysed to exclude unspecific cytotoxic or antioxidant effects. Constituents of the extracts were characterized by bioassay-guided fractionation and by analysis using gas or liquid chromatography coupled to mass spectrometric (Orbitrap) analysis.ResultsExtracts of Plectranthus zeylanicus prepared with n-hexane or dichloromethane potently suppressed 5-LO activity in stimulated human neutrophils (IC50=6.6 and 12 µg/ml, respectively) and inhibited isolated human recombinant 5-LO (IC50=0.7 and 1.2 µg/ml, respectively). In contrast, no significant radical scavenging activity or suppression of ROS formation was observed, and neutrophil viability was unaffected. Besides ubiquitously occurring ingredients, coleone P, cinncassiol A and C, and callistric acid were identified as constituents in the most active fraction.ConclusionsTogether, potent inhibition of 5-LO activity, without concomitant anti-oxidant activity and cytotoxic effects, rationalizes the ethnopharmacological use of Plectranthus zeylanicus as anti-inflammatory remedy. Modern chromatographic/mass spectrometric analysis reveals discrete chemical structures of relevant constituents.
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Journal: Journal of Ethnopharmacology - Volume 151, Issue 2, 3 February 2014, Pages 800–809