کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2545469 1123955 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Decoctions from Citrus reticulata Blanco seeds protect the uroepithelium against Escherichia coli invasion
موضوعات مرتبط
علوم پزشکی و سلامت داروسازی، سم شناسی و علوم دارویی داروشناسی
پیش نمایش صفحه اول مقاله
Decoctions from Citrus reticulata Blanco seeds protect the uroepithelium against Escherichia coli invasion
چکیده انگلیسی

Ethnopharmacological relevanceTraditional usage suggests Citrus reticulata Blanco seeds have beneficial effects against infection. The purpose of this study was to investigate the effect of Citrus reticulata on the uroepithelium and to determine the mechanisms responsible for protection against urinary tract infection (UTI).Materials and methodsHuman bladder cell lines T24 and 5637 were employed in a cell culture infection model to determine the effects of Citrus reticulata treatment on Escherichia coli adherence and invasion of the uroepithelium. β1 integrin and caveolin-1 mRNA expression was assessed using RT real-time PCR. β1 integrin protein expression was confirmed by Western Blot. The effect of Citrus reticulata on bacteria was investigated using antibacterial sensitivity, yeast agglutination and biofilm assays.ResultsCitrus reticulata treatment decreased β1 integrin expression and reduced bacterial invasion while adhesion of uroepithelial cells was not affected. Caveolin-1 expression was not influenced either and Citrus reticulata did neither exhibit any direct antimicrobial effect nor interfered with type 1 fimbriae binding.ConclusionsOur results show that Citrus reticulata has a protective effect on the uroepithelium as seen by reduced bacterial invasion of uroepithelial cells. These properties suggest that seeds from Citrus reticulata may have therapeutic potential in preventing UTI.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Ethnopharmacology - Volume 150, Issue 2, 25 November 2013, Pages 770–774
نویسندگان
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