Ostreae Testa prevent ovariectomy-induced bone loss in mice by osteoblast activations

Aim of the studyThe anti-osteoporotic effect of heated powder of Ostreae Testa (hPOT), a powder of oyster shell—Ostrea gigas Thumb (Ostrediae) was observed in vitro and in vivo.Materials and methodsThe effects on proliferation and alkaline phosphatase activity of primary osteoblasts, bone nodule formation, pit formation of osteoclasts and osteoclastogenesis were observed in vitro, and to observe the in vivo efficacy hPOT was orally administered once a day for 28 days to bilateral ovariectomy-induced osteoporosis mice at 125, 250 and 500 mg/kg (of body weight).ResultsAlthough hPOT did not influence the pit formation and the number of multinucleated osteoclast-like cells, osteoclastogenesis, it enhanced the proliferation of primary osteoblasts, differentiation (ALP activity) and bone nodule formation of osteoblast in vitro. The estrogen-deficient osteoporotic changes were dramatically prevented by hPOT treatment except for osteoclasts/bone perimeter.ConclusionsIn conclusion, hPOT prevents OVX-induced osteoporosis through osteoblasts activation effects.