کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2569153 1128513 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Apoptosis induction by silica nanoparticles mediated through reactive oxygen species in human liver cell line HepG2
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Apoptosis induction by silica nanoparticles mediated through reactive oxygen species in human liver cell line HepG2
چکیده انگلیسی

Silica nanoparticles are increasingly utilized in various applications including agriculture and medicine. In vivo studies have shown that liver is one of the primary target organ of silica nanoparticles. However, possible mechanisms of hepatotoxicity caused by silica nanoparticles still remain unclear. In this study, we explored the reactive oxygen species (ROS) mediated apoptosis induced by well-characterized 14 nm silica nanoparticles in human liver cell line HepG2. Silica nanoparticles (25–200 μg/ml) induced a dose-dependent cytotoxicity in HepG2 cells. Silica nanoparticles were also found to induce oxidative stress in dose-dependent manner indicated by induction of ROS and lipid peroxidation and depletion of glutathione (GSH). Quantitative real-time PCR and immunoblotting results showed that both the mRNA and protein expressions of cell cycle checkpoint gene p53 and apoptotic genes (bax and caspase-3) were up-regulated while the anti-apoptotic gene bcl-2 was down-regulated in silica nanoparticles treated cells. Moreover, co-treatment of ROS scavenger vitamin C significantly attenuated the modulation of apoptotic markers along with the preservation of cell viability caused by silica nanoparticles. Our data demonstrated that silica nanoparticles induced apoptosis in human liver cells, which is ROS mediated and regulated through p53, bax/bcl-2 and caspase pathways. This study suggests that toxicity mechanisms of silica nanoparticles should be further investigated at in vivo level.


► We explored the mechanisms of toxicity caused by silica NPs in human liver HepG2 cells.
► Silica NPs induced a dose-dependent cytotoxicity in HepG2 cells.
► Silica NPs induced ROS generation and oxidative stress in a dose-dependent manner.
► Silica NPs were also modulated apoptosis markers both at mRNA and protein levels.
► ROS mediated apoptosis induced by silica NPs was preserved by vitamin C.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 259, Issue 2, 1 March 2012, Pages 160–168
نویسندگان
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