کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2569291 1128521 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Protective effects of melittin on transforming growth factor-β1 injury to hepatocytes via anti-apoptotic mechanism
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Protective effects of melittin on transforming growth factor-β1 injury to hepatocytes via anti-apoptotic mechanism
چکیده انگلیسی

Melittin is a cationic, hemolytic peptide that is the main toxic component in the venom of the honey bee (Apis mellifera). Melittin has multiple effects, including anti-bacterial, anti-viral and anti-inflammatory, in various cell types. However, the anti-apoptotic mechanisms of melittin have not been fully elucidated in hepatocytes. Apoptosis contributes to liver inflammation and fibrosis. Knowledge of the apoptotic mechanisms is important to develop new and effective therapies for treatment of cirrhosis, portal hypertension, liver cancer, and other liver diseases. In the present study, we investigated the anti-apoptotic effect of melittin on transforming growth factor (TGF)-β1-induced apoptosis in hepatocytes. TGF-β1-treated hepatocytes were exposed to low doses (0.5 and 1 μg/mL) and high dose (2 μg/mL) of melittin. The low doses significantly protected these cells from DNA damage in TGF-β1-induced apoptosis compared to the high dose. Also, melittin suppressed TGF-β1-induced apoptotic activation of the Bcl-2 family and caspase family of proteins, which resulted in the inhibition of poly-ADP-ribose polymerase (PARP) cleavage. These results demonstrate that TGF-β1 induces hepatocyte apoptosis and that an optimal dose of melittin exerts anti-apoptotic effects against TGF-β1-induced injury to hepatocytes via the mitochondrial pathway. These results suggest that an optimal dose of melittin can serve to protect cells against TGF-β1-mediated injury.


► We investigated the anti-apoptotic effect of melittin on TGF-β1-induced hepatocyte.
► TGF-β1 induces hepatocyte apoptosis.
► TGF-β1-treated hepatocytes were exposed to low doses and high dose of melittin.
► Optimal dose of melittin exerts anti-apoptotic effects to hepatocytes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 256, Issue 2, 15 October 2011, Pages 209–215
نویسندگان
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