کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2572004 | 1128664 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lack of activity of cadmium in in vitro estrogenicity assays
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
EGFchlorophenol red-β-d-galactopyranosideCPRGProliferative effectCdCl2TCASRBFBSDMEM17β-estradiol - 17β استرادیولMAPK - MAPKDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoEndocrine disrupter - اختلال غدد درون ریزEstrogen - استروژنtrichloroacetic acid - اسید ترشکلراکتیکHER - اوYES - بلهfetal bovine serum - سرم جنین گاوYeast Estrogen Screen - صفحه نمایش استروئید مخمرE-screen - صفحه نمایش الکترونیکیepidermal growth factor - عامل رشد اپیدرمیMetallothionein - متالوتونیئینSignalling pathways - مسیرهای سیگنالینگHeavy metal - هوی متال، فلز سنگینmitogen activated protein kinase - پروتئین کیناز فعال Mitogen فعال استCadmium - کادمیمCadmium chloride - کلرید کادمیومMAP kinases - کیناز MAPEstrogen receptor - گیرنده استروژنHuman estrogen receptor - گیرنده استروژن انسان
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Prompted by reports about strong estrogenic effects of cadmium, attempts were made to reproduce these observations using the yeast estrogen screen (YES) and the E-Screen assays. For the first time, possible activation of the Src/MAPK pathway was also investigated. In the YES, only a slight activation (10% of a maximal effect) of the estrogen receptor alpha (ERα) was observed at cadmium concentrations between 5 Ã 10â7 M and 5 Ã 10â6 M. In the E-Screen assay, carried out by two laboratories, the heavy metal was without observable cell proliferative effects when tested in the range between 6 Ã 10â11 M and 1 Ã 10â5 M. However, in both assays, cadmium led to a reduction of the effects of 17β-estradiol (E2). Treatment of MCF-7 human breast cancer cells with 1 Ã 10â7 M cadmium failed to induce phosphorylation of Src and the MAP kinases Erk1 and Erk2-effects shown to occur with E2 and epidermal growth factor (EGF). In summary, we were unable to confirm the strong estrogenicity of cadmium reported recently by a number of laboratories. This apparent absence of effects in our hands is not due to a lack of uptake of the metal or to effective protection against cadmium by high levels of glutathione or metallothionein, since toxicity and an antagonism of E2 responses were observed both in the YES and the E-Screen.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology and Applied Pharmacology - Volume 216, Issue 1, 1 October 2006, Pages 20-28
Journal: Toxicology and Applied Pharmacology - Volume 216, Issue 1, 1 October 2006, Pages 20-28
نویسندگان
Elisabete Silva, Maria José Lopez-Espinosa, José-Manuel Molina-Molina, Marieta Fernández, Nicolas Olea, Andreas Kortenkamp,