Inhibitory Effects of Radix Salviae Miltiorrhizae Extract-F on Ethanol-Induced Gastric Mucosal Lesion and Gastric Mucosal Blood Vessels Impairment and Its Effects on Lipid Peroxidation in Rats

ObjectiveThe inhibitory effects of Radix Salviae Miltiorrhizae Extract-F (RSME-F) on ethanol-induced gastric mucosal injury and microvascular injury and its effects on lipid peroxidation in rats were investigated.MethodsIn the study, 160–200 g Wistar male rats were randomly divided into different groups, including the blank control, the control, and RSME-F. 24-hour fasting was arranged before experiment, though water was allowed. Saline, ethanol, and RSME-F (1mL/100g) were fed to the rats respectively. 0.5 hour later the rats were sacrificed. Gastric mucosal lesion, gastric mucosal microvascular injury, gastric activity of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity, and content of gastric mucosal malondialdehyde (MDA) were measured.ResultsCompared with the control group, the RSME-F group showed a decrease in gastric mucosal lesion (2.53 ± 0.50 vs 7.57 ± 0.22, P < 0.01) and gastric mucosal injury depth (Class III injury were 20.67 ± 11.20 vs 51.88 ± 5.05, P < 0.01). Evan's Blue in the gastric mucosa and Monostral Blue stain area were 8.05 ± 1.86 vs 12.46 ± 0.70 (ng/g wet weight) P<0.05 and 0.90 ± 0.10 vs 4.54 ± 0.83 (%), P <0.05, respectively. In addition, compared with the blank control group, the control group showed reduced SOD (Nu/mg pro) and GSH-Px (u) activity of gastric mucosa with 3.18 ± 0.59 vs 7.68 ± 0.68 (Nu/mg pro, P<0.01) and 31.52 ± 14.65 vs 52.67 ± 21.32 (u, P<0.01), respectively, but the SOD and GSH-Px activity in gastric mucosa of the RSME-F group was 6.11 ± 0.54 (Nu/mg pro) and 49.31 ± 15.82 (u), respectively, which was significantly higher than that of control group (P<0.01). Compared with control group, the content of gastric mucosal MDA in RSME-F group was 0.92 ± 0.06 vs 1.38 ± 0.19 (nmol/mg pro, P<0.05).ConclusionIt is believed that RSME-F inhibited gastric mucosal lesion and microvascular injury induced by ethanol. In addition, it played a role in promoting the gastric mucosal SOD and GSH-Px activity and in reducing the content of gastric mucosal MDA.