A social recognition test for female mice reveals behavioral effects of developmental chlorpyrifos exposure

CD-1 mice were exposed to the organophosphate pesticide chlorpyrifos (CPF) both prenatally (gestational days 15-18; doses 0, 3 or 6 mg/kg) and postnatally (postnatal days 11-14, doses 0, 1 or 3 mg/kg). When four-month-olds, females underwent a social recognition test in which ultrasound vocalizations (USVs) and social investigation behavior emitted by a resident female in the presence of a female partner were measured during two subsequent 3 min sessions (interval between the two sessions 45 min). Throughout the social recognition test a marked increase in USVs was found in females prenatally treated with the highest CPF dose; USV increase was also paralleled by a selective increase in frequency and not in duration of social investigation. These results confirm that developmental exposure to CPF induces long-lasting alterations in the social behavior repertoire of the mouse, thus extending our previous observations on the effects of postnatal CPF on male agonistic behavior to the female sex. They also suggest that social recognition can be easily and rapidly assessed in the female mouse making it possible to evaluate, primarily by means of USV emission, even subtle alteration of social behavioral patterns dissociated from cognitive components of individual recognition.