کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2593556 | 1562176 | 2014 | 11 صفحه PDF | دانلود رایگان |
• DNB induces the testicular toxicity in Sprague–Dawley rats.
• We identified key genes and proteins related to early testicular toxicity.
• Genes related to germ/Sertoli cell junction were identified during early testicular toxicity.
• Proteins related to apoptosis were also identified during early testicular toxicity.
1,3-Dinitrobenzene (DNB) is an industrial intermediate and testicular toxicant that has been shown to target Sertoli cells. The mechanism of action of DNB in the testis, however, is unclear. To investigate global alterations in gene or protein expression during testicular toxicity, testes from rats treated orally with DNB were subjected to microarray and two-dimensional gel electrophoresis (2-DE) analyses. Histopathological abnormalities were detected in the testes of the DNB-treated rats. Microarray analysis revealed that, during early testicular toxicity, several genes involved in apoptosis, germ cell/Sertoli cell junction, and tight junction signaling pathways were differentially expressed. Based on 2-DE analysis, 36 protein spots showing significantly different expression during early testicular toxicity were selected and identified. Network analysis of the identified proteins revealed that these proteins are associated with cellular development or reproductive system diseases. Collectively, these data will help clarify the molecular mechanism underlying testicular toxicity in DNB-exposed rats.
Journal: Reproductive Toxicology - Volume 43, January 2014, Pages 45–55