کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2593595 1562181 2013 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Reversible effect of developmental exposure to chlorpyrifos on late-stage neurogenesis in the hippocampal dentate gyrus in mouse offspring
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Reversible effect of developmental exposure to chlorpyrifos on late-stage neurogenesis in the hippocampal dentate gyrus in mouse offspring
چکیده انگلیسی

The effect of developmental exposure to chlorpyrifos (CPF) on hippocampal neurogenesis was examined in male mice after maternal dietary exposure to CPF at 0, 4, 20, or 100 ppm from gestation day 10 to postnatal day (PND) 21. Cholinesterase activity was dose-dependently decreased in red blood cells at ≥4 ppm and in the brain at 100 ppm both in dams and offspring on PND 21. Immunohistochemically, doublecortin+ cells were decreased at ≥20 ppm in the subgranular zone (SGZ) of the dentate gyrus, and NeuN+-expressing mature neurons were decreased at 100 ppm in the hilus on PND 21. There were no differences in the numbers of progenitor populations expressing Tbr2 or M1 muscarinic acetylcholine receptors. Transcript levels of Dcx also decreased at ≥20 ppm, and those of Pcna, Casp3, Bax, Bcl2, Pax6 and Tbr2 were unchanged in the dentate gyrus by real-time RT-PCR. At PND 77, hippocampal neurogenesis was unchanged. These results suggest that developmental CPF exposure directly but transiently suppresses maturation of late-stage granule cell lineages in the SGZ and affects interneuron populations in the hilus.


► We examined developmental exposure effect of chlorpyrifos on neurogenesis in mice.
► ChE activity in RBC of dams and offspring was decreased at ≥4 ppm CPF.
► ChE activity in the brain of dams and offspring was decreased at 100 ppm CPF.
► CPF transiently suppressed late-stage granule cell maturation in the SGZ.
► Cholinergic stimulation was unrelated to the suppressed neurogenesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Toxicology - Volume 38, July 2013, Pages 25–36
نویسندگان
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