کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2594002 1132255 2011 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of multiple doses of cyclophosphamide on mouse testes: Accessing the germ cells lost, and the functional damage of stem cells
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم محیط زیست بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
Effects of multiple doses of cyclophosphamide on mouse testes: Accessing the germ cells lost, and the functional damage of stem cells
چکیده انگلیسی

Spermatogenesis is sensitive to the chemotherapeutic drug cyclophosphamide, which decreases the patients’ sperm count. Since the recovery of fertility is dependent on regeneration from stem cells, in the present study we evaluated the ability of cyclophosphamide-exposed stem spermatogonia from mice to regenerate spermatogenesis in situ and after transplantation. When seven doses of cyclophosphamide were given at 4-day intervals, the differentiating germ cells were largely eliminated but ∼50% of the undifferentiated type A spermatogonia remained. We monitored the recovery and found that sperm production recovered to 64% of control within the time expected. When the cyclophosphamide-surviving spermatogonia were transplanted into recipient mice, recovery of spermatogenesis from the cyclophosphamide-exposed donor cells was observed, but was reduced when compared to cells from cryptorchid donors. Thus, multidose regimens of cyclophosphamide did not eliminate the stem spermatogonia, but resulted in cell loss and residual damage.


► Effects of cyclophosphamide in stem spermatogonia.
► Recovery of spermatogenesis evaluated in situ and after transplantation.
► Stem cells showed good recovery in situ.
► Poor recovery after transplantation.
► Cyclophosphamide caused stem cell depletion and functional damage.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Toxicology - Volume 32, Issue 4, December 2011, Pages 395–406
نویسندگان
, , , , , ,