کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2594638 | 1132275 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: In vitro exposure to environmental tobacco smoke induces CYP1B1 expression in human luteinized granulosa cells In vitro exposure to environmental tobacco smoke induces CYP1B1 expression in human luteinized granulosa cells](/preview/png/2594638.png)
Women smokers and women exposed to environmental tobacco smoke (ETS) have reduced ovarian function as evidenced by an earlier menopause, reduced follicular numbers, decreased levels of circulating estradiol, and decreased conception rates; however, the mechanism of action of altered ovarian function by ETS is poorly understood. The direct effects of ETS were evaluated using human luteinized granulosa cells (HLGCs) exposed to ETS in primary cell culture. Exposure to ETS caused a decrease in both estradiol and progesterone production. There was a concentration dependent increase in CYP1B1 gene and protein expression without a change in catechol-O-methyltransferase (COMT) expression. This is the first report of CYP1B1 induction secondary to ETS exposure in cells from the human ovary. CYP1B1 metabolizes both endogenous estrogens and polyaromatic hydrocarbons in ETS to a variety of reactive species and may contribute to the complex effects of ETS on ovarian function.
Journal: Reproductive Toxicology - Volume 22, Issue 4, November 2006, Pages 731–737